一种使用银染和胆碱酯酶联合染色法对骨骼肌整装标本中的轴突和终板进行可视化的方法。

A method for visualizing axons and endplates throughout whole mounts of skeletal muscles using combined silver and cholinesterase stain.

作者信息

Slack J R, Pockett S

出版信息

Pflugers Arch. 1981 Oct;391(4):306-8. doi: 10.1007/BF00581512.

Abstract

A silver-cholinesterase stain is described which allows the visualization of the complete axonal tree, perineurial sheaths, motor nerve terminals and the cholinesterase at neuromuscular junctions throughout whole mounts of relatively thin muscles. The method is rapid, reliable and simple and allows the preservation of topographical information regarding both terminal and nodal sprouting in the whole muscle.

摘要

本文描述了一种银胆碱酯酶染色法，该方法可使相对较薄肌肉的整个标本中完整的轴突树、神经束膜、运动神经末梢以及神经肌肉接头处的胆碱酯酶可视化。该方法快速、可靠且简单，能够保留整个肌肉中关于终末和节段性发芽的地形学信息。