小鼠基因组位点的核苷酸序列，包括U6（4.8S）核RNA的一个基因或假基因。
Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.
作者信息
Ohshima Y, Okada N, Tani T, Itoh Y, Itoh M
出版信息
Nucleic Acids Res. 1981 Oct 10;9(19):5145-58. doi: 10.1093/nar/9.19.5145.
Abstract
We have isolated four clones which hybridize with U6 (4.8S) nuclear RNA, a mammalian small nuclear RNA(nRNA), from DNA of BALB/C mouse liver. Their restriction maps are totally different from each other, indicating that they derived from different loci in the mouse genome. The nucleotide sequences around the hybridizing region in the three clones have been determined. One clone gives a gene that is co-linear with the U6 RNA. There is a sequence TATAAAT beginning 31 nucleotides upstream of the gene, which may suggest that the U6 RNA is transcribed by RNA polymerase II. The other two clones contain a pseudogene for the U6 RNA which has 7 or 9 nucleotide changes from the RNA. The pseudogenes are surrounded by radically different sequences from those surrounding the gene, and they are closely linked to a pseudogene for another snRNA, 4.5S-I RNA, or a part of highly repetitive an interspersed sequence B1.
摘要
我们从BALB/C小鼠肝脏的DNA中分离出了四个与U6（4.8S）核RNA（一种哺乳动物小核RNA，即snRNA）杂交的克隆。它们的限制性图谱彼此完全不同，这表明它们源自小鼠基因组中的不同位点。已经确定了三个克隆中杂交区域周围的核苷酸序列。一个克隆给出了一个与U6 RNA共线性的基因。在该基因上游31个核苷酸处有一个TATAAAT序列，这可能表明U6 RNA是由RNA聚合酶II转录的。另外两个克隆包含一个U6 RNA的假基因，该假基因与RNA有7个或9个核苷酸的变化。这些假基因周围的序列与基因周围的序列截然不同，并且它们与另一个snRNA 4.5S-I RNA的假基因或高度重复的散布序列B1的一部分紧密相连。
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