DePalatis L R, Fiorindo R P, Ho R H
Endocrinology. 1982 Jan;110(1):282-4. doi: 10.1210/endo-110-1-282.
The aim of this study was to demonstrate the presence of Substance P (SP) immunoreactive cells within the anterior pituitary gland of the adult guinea pig. By utilizing the PAP technique of Sternberger, immunoreactive SP-containing cells were visualized in all animals studied. These cells were most dense in the ventral portion of the gland. When adjacent tissue sections were incubated with anti-SP serum and anti-rat TSH serum, it was observed that most, if not all, the SP immunoreactive cells examined also exhibited TSH immunoreactivity. However, not all TSH immunoreactive cells contained SP immunoreactivity. Immunoabsorption controls indicated that the immunohistochemical staining reactions were specific for both SP and TSH. The results of this study indicate that immunoreactive SP is present in the guinea pig anterior pituitary and that this peptide is localized within TSH immunoreactive cells.
本研究的目的是证实成年豚鼠垂体前叶内P物质（SP）免疫反应性细胞的存在。通过使用Sternberger的PAP技术，在所研究的所有动物中均可见到含免疫反应性SP的细胞。这些细胞在腺体腹侧部分最为密集。当相邻组织切片用抗SP血清和抗大鼠促甲状腺激素（TSH）血清孵育时，观察到所检测的大多数（如果不是全部）SP免疫反应性细胞也表现出TSH免疫反应性。然而，并非所有TSH免疫反应性细胞都含有SP免疫反应性。免疫吸收对照表明，免疫组织化学染色反应对SP和TSH均具有特异性。本研究结果表明，豚鼠垂体前叶存在免疫反应性SP，且该肽定位于TSH免疫反应性细胞内。
