Mosmann T R, Longenecker B M
J Immunol. 1982 Jan;128(1):100-4.
Background responses have been assessed by fusing lipopolysaccharide- (LPS) stimulated spleen cells from unimmunized mice with MOPC 315.43 myeloma cells and screening the hybrids for the production of antibody against chicken red blood cells (CRBC). Clones specific for CRBC represented about 1% of total hybrid clones (1000 to 5000 clones were obtained per mouse). The majority of the anti-CRBC clones (greater than 95%) secreted antibody against polymorphic CRBC determinants (present on CRBC from some but not all chickens) rather than species-specific determinants present on all CRBC. Some of the polymorphic determinants were linked to the B locus (the MHC of the chicken) and some were non-B antigens. The relative amount of these 2 categories varied slightly according to the mouse strain. These results agree well with the specificities of natural mouse antibody and rosette-forming spleen cells. The response of immunized mice against CRBC and human RBC was also selective for polymorphic determinants. These results have considerable importance for the use of xenogeneic RBC as "standard" antigens, and are interpreted in terms of a model for the advantages of genetic polymorphism as a protection against antigen mimicry by parasites.
通过将来自未免疫小鼠的脂多糖（LPS）刺激的脾细胞与MOPC 315.43骨髓瘤细胞融合，并筛选杂交细胞产生抗鸡红细胞（CRBC）抗体，评估了背景反应。对CRBC特异的克隆约占杂交克隆总数的1%（每只小鼠获得1000至5000个克隆）。大多数抗CRBC克隆（超过95%）分泌针对多态性CRBC决定簇（存在于部分而非所有鸡的CRBC上）的抗体，而非所有CRBC上都存在的种属特异性决定簇。一些多态性决定簇与B位点（鸡的主要组织相容性复合体）相关，一些是非B抗原。这两类的相对量根据小鼠品系略有不同。这些结果与天然小鼠抗体和形成玫瑰花结的脾细胞的特异性非常吻合。免疫小鼠对CRBC和人红细胞的反应也对多态性决定簇具有选择性。这些结果对于将异种红细胞用作“标准”抗原有相当重要的意义，并根据遗传多态性作为抵御寄生虫抗原模拟的优势模型进行了解释。
