Chang T W, Testa D, Kung P C, Perry L, Dreskin H J, Goldstein G
J Immunol. 1982 Feb;128(2):585-9.
OKT3 monoclonal antibody, a human T cell mitogen, induced interferon production by cultured mononuclear cells at 10(-11) M concentrations. Interferon was secreted only under conditions wherein OKT3 was mitogenic, and production was correlated with cell proliferation. Thus, like mitogenesis, interferon secretion reached a peak 3 days after OKT3 stimulation, was inhibited by a factor(s) in human serum, and required 1000 times higher concentrations of Fab and F(ab')2 fragments of OKT3 for induction. The interferon was most likely of "gamma" (immune) type, because pH 2 and 56 degrees C treatments denatured it, whereas anti-alpha or -beta interferon antibodies did not. Mononuclear cells were fractionated into subpopulations that contained OKT4+ cells (helper/inducer T cells), OKT8+ cells (cytotoxic/suppressor T cells), and OKM1+ cells (monocytes) by combining sheep red blood cell rosetting and complement-mediated lysis using monoclonal antibodies against specific cell types. Both OKT4+ and OKT8+ cells proliferated upon OKT3 stimulation with the absolute requirement of OKM1+ cells. However, OKT4+ cells plus OKM1+ cells were necessary for the secretion of interferon. Studies with selective pretreatments with mitomycin C suggested that gamma-interferon was secreted by the OKT4+ cells and that the OKM1+ population subserved an accessory function.
OKT3单克隆抗体，一种人类T细胞促有丝分裂原，在浓度为10(-11)M时可诱导培养的单核细胞产生干扰素。干扰素仅在OKT3具有促有丝分裂作用的条件下分泌，且其产生与细胞增殖相关。因此，与有丝分裂一样，干扰素分泌在OKT3刺激后3天达到峰值，受到人血清中某种因子的抑制，并且诱导所需的OKT3的Fab和F(ab')2片段浓度要高1000倍。该干扰素很可能是“γ”（免疫）型，因为pH 2和56℃处理会使其变性，而抗α或β干扰素抗体则不会。通过将绵羊红细胞玫瑰花结形成与使用针对特定细胞类型的单克隆抗体进行补体介导的裂解相结合，将单核细胞分离成包含OKT4+细胞（辅助/诱导性T细胞）、OKT8+细胞（细胞毒性/抑制性T细胞）和OKM1+细胞（单核细胞）的亚群。OKT3刺激后，OKT4+和OKT8+细胞均会增殖，且绝对需要OKM1+细胞。然而，干扰素的分泌需要OKT4+细胞加OKM1+细胞。用丝裂霉素C进行选择性预处理的研究表明，γ干扰素由OKT4+细胞分泌，而OKM1+群体发挥辅助功能。
