组织学和组织化学光学显微镜与扫描电子显微镜的一一对应关系。

One to one correlation of histological and histochemical light microscopy with scanning electron microscopy.

作者信息

Sweney L R, Shapiro B L

出版信息

Scan Electron Microsc. 1981(Pt 2):63-72.

Abstract

Several histological stains were applied to specimens after scanning electron microscopic (SEM) processing. Histochemical stains were applied before SEM fixation. After staining, the specimens were processed and dried by SEM techniques. The specimens were taped to a microslide, specimen side up, scribed and covered with immersion oil. After light micrography (LM), the oil was removed and the specimens mounted and gold coated. The same cells were then relocated and photographed by SEM. Periodic acid Schiff was not usable as a specific tissue aldehyde stain, but did prove to be a useful counterstain for dehydrogenase stained specimens. Colloidal iron, as seen by SEM, resulted in a non-specific granular deposit over the specimen and the substrate. All the other histological stains examined--alcian blue, Grams, Feulgen and toluidine blue--were specific and did not change the specimen ultrastructure. The histochemical stains--acid and alkaline phosphatase and three dehydrogenases--were also specific. There were some minor ultrastructural changes with the AcPase and AlPase stains. However, the tissue surface was not significantly distorted. These and other techniques should prove to be a useful adjunct to SEM studies.

摘要

在扫描电子显微镜（SEM）处理后，对标本应用了几种组织学染色剂。组织化学染色在SEM固定之前进行。染色后，标本通过SEM技术进行处理和干燥。将标本贴在载玻片上，标本面朝上，划线并覆盖浸油。在进行光学显微镜（LM）观察后，去除油，将标本安装并镀金。然后用SEM重新定位并拍摄相同的细胞。过碘酸希夫染色不能用作特定的组织醛染色，但被证明是脱氢酶染色标本的有用复染剂。通过SEM观察，胶体铁在标本和底物上产生非特异性颗粒沉积物。所有其他检查的组织学染色剂——阿尔辛蓝、革兰氏染色、福尔根染色和甲苯胺蓝——都是特异性的，并且不会改变标本的超微结构。组织化学染色剂——酸性和碱性磷酸酶以及三种脱氢酶——也是特异性的。酸性磷酸酶（AcPase）和碱性磷酸酶（AlPase）染色有一些轻微的超微结构变化。然而，组织表面没有明显变形。这些技术和其他技术应被证明是SEM研究的有用辅助手段。