Ts'ao C H, Krajewski D V
Am J Pathol. 1982 Jan;106(1):1-7.
Dextran has been shown to alter the mechanical properties and lysability of fibrin. The present study was undertaken to determine whether it would also influence the ability of polymerizing fibrin to activate platelets. Human fibrinogen, with or without the presence of dextran, was incubated with either human thrombin or reptilase at 37 C. Macroscopically evident fibrils first appeared at 7 1/2 to 8 minutes in fibrinogen solution not containing dextran and at 2 1/2 to 3 minutes in solution containing dextran. Both solutions caused aggregation of washed human platelets, but the one containing dextran was less potent (P less than 0.005). Similarly, polymerizing fibrin, under the influence of dextran, was a less potent stimulator of platelet release of labeled serotonin. Scanning electron microscopy showed decreased platelet adhesion to fibrin polymerized in the presence of dextran. Inclusion of dextran in plasminogen-rich bovine fibrin clots facilitated their lysis by urokinase. Apparently, dextran not only increases the lysability of fibrin but also alters the platelet-activating activity of polymerizing fibrin.
已证明葡聚糖可改变纤维蛋白的机械性能和可溶解性。本研究旨在确定它是否也会影响聚合纤维蛋白激活血小板的能力。将含有或不含葡聚糖的人纤维蛋白原与人类凝血酶或蛇毒凝血酶在37℃下孵育。在不含葡聚糖的纤维蛋白原溶液中，肉眼可见的纤维在7.5至8分钟时首次出现，而在含葡聚糖的溶液中则在2.5至3分钟时出现。两种溶液均导致洗涤后的人血小板聚集，但含葡聚糖的溶液作用较弱（P小于0.005）。同样，在葡聚糖的影响下，聚合纤维蛋白对标记血清素的血小板释放刺激作用较弱。扫描电子显微镜显示，血小板对在葡聚糖存在下聚合的纤维蛋白的粘附减少。在富含纤溶酶原的牛纤维蛋白凝块中加入葡聚糖有助于尿激酶对其进行溶解。显然，葡聚糖不仅增加了纤维蛋白的可溶解性，还改变了聚合纤维蛋白的血小板激活活性。
