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母血中甲胎蛋白的测定:三种市售放射免疫分析试剂盒与两种非市售放射免疫分析方法的比较

Measurement of alpha-fetoprotein in maternal serum: three commercial radioimmunoassay kits and two non-commercial radioimmunoassays compared.

作者信息

Forest J C, Verreault F, Pouliot M

出版信息

Clin Chem. 1982 Feb;28(2):339-43.

PMID:6173148
Abstract

We evaluated three commercial radioimmunoassay kits (Amersham, Dainabot, Clinical Assays) and two non-commercial methods for determining alpha-fetoprotein in maternal serum during pregnancy. All five procedures were found to be acceptable with respect to practicability, sensitivity, linearity, and precision. Similar results were obtained with Dainabot, Clinical Assays, and the two non-commercial methods, but the Amersham method revealed a proportional error, results being about 20% lower than those by the other methods. Use of the international unit system is suggested for reporting results for AFP, to facilitate comparison between methods and laboratories.

摘要

我们评估了三种商用放射免疫分析试剂盒(阿默沙姆、戴纳博特、临床检验)以及两种非商用方法,用于测定孕期母血中的甲胎蛋白。就实用性、灵敏度、线性和精密度而言,所有这五种方法均被认为是可接受的。戴纳博特、临床检验以及两种非商用方法得出了相似的结果,但阿默沙姆方法显示出比例误差,其结果比其他方法低约20%。建议使用国际单位制来报告甲胎蛋白的结果,以便于不同方法和实验室之间进行比较。

相似文献

1
Measurement of alpha-fetoprotein in maternal serum: three commercial radioimmunoassay kits and two non-commercial radioimmunoassays compared.母血中甲胎蛋白的测定:三种市售放射免疫分析试剂盒与两种非市售放射免疫分析方法的比较
Clin Chem. 1982 Feb;28(2):339-43.
2
Evaluation of four "kit" immunoassay methods for determination of alpha-fetoprotein in serum during pregnancy.四种“试剂盒”免疫分析方法用于孕期血清甲胎蛋白测定的评估。
Clin Chem. 1979 Nov;25(11):1905-8.
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Commercial radioimmunoassay kit for measurement of alpha-fetoprotein adapted for use with dried blood specimens from newborns.用于测量甲胎蛋白的商用放射免疫分析试剂盒,适用于新生儿干血标本。
Clin Chem. 1982 May;28(5):1207-10.
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Evaluation of a commercial immunoenzymometric assay for alpha-fetoprotein.一种用于甲胎蛋白的商业免疫酶联免疫分析方法的评估。
Clin Chem. 1985 Nov;31(11):1868-70.
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Evaluation of an enhanced luminescence assay for alpha-fetoprotein.甲胎蛋白增强发光检测法的评估
Clin Chem. 1986 Nov;32(11):2066-9.
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[Radioimmunoassay of serum alpha-fetoprotein using Tachisorb as a rapid separation reagent].[以Tachisorb作为快速分离试剂的血清甲胎蛋白放射免疫测定法]
Radioisotopes. 1983 Nov;32(11):563-6.
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[Fundamental and clinical evaluation of "AMERWELL AFP RIA-kit" for radioimmunoassay of alpha-fetoprotein].“AMERWELL甲胎蛋白放射免疫分析试剂盒”用于甲胎蛋白放射免疫分析的基础与临床评价
Kaku Igaku. 1988 Oct;25(10):1171-8.
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[Determination of alpha-fetoprotein (AFP) by "Eiken" RIA kit (author's transl)].用“荣研”放射免疫分析试剂盒测定甲胎蛋白(作者译)
Radioisotopes. 1978 Feb;27(2):101-4. doi: 10.3769/radioisotopes.27.2_101.
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[Measurement of alpha-fetoprotein by radioimmunoassay using polyethylene glycol (author's transl)].用聚乙二醇放射免疫分析法测定甲胎蛋白(作者译)
Kaku Igaku. 1979 Dec;16(8):1425-30.
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[Fundamental and clinical evaluation of "ELSA AFP RIA kit" for radioimmunoassay of alpha-fetoprotein].甲胎蛋白放射免疫分析“ELSA AFP RIA试剂盒”的基础与临床评价
Kaku Igaku. 1985 Dec;22(12):1851-6.

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Fluoroimmunoassay: an alternative to radioimmunoassay?荧光免疫测定法:放射免疫测定法的替代方法?
Eur J Nucl Med. 1987;13(8):397-401. doi: 10.1007/BF00292490.