Muñoz A, Carrasco L
Intervirology. 1981;16(2):106-13. doi: 10.1159/000149254.
The induction kinetics of the antiviral state in HeLa cells treated with human lymphoblastoid interferon (IFN) was studied. In cells treated with 4-200 U/ml IFN, the antiviral state was fully established in 7-9 h. Inhibition of virus multiplication was more rapid if the concentration of IFN was increased to 1,000 U/ml. This antiviral state gradually disappeared during the 48 h after IFN removal. Several compounds known to act on the cell membrane or on the cytoskeleton were tested for their influence on the establishment and reversal of the antiviral state. None of them were found to influence these two parameters to a significant extent. In contrast, placing HeLa cells in medium lacking NaCl partially reversed the blockade to virus multiplication induced by IFN treatment. Cells treated with IFN and later placed in hypotonic medium synthesized virus proteins after encephalomyocarditis virus infection, although at a reduced level compared to cells that had not been treated with IFN.
研究了用人淋巴母细胞干扰素（IFN）处理的HeLa细胞中抗病毒状态的诱导动力学。在用4 - 200 U/ml IFN处理的细胞中，抗病毒状态在7 - 9小时内完全建立。如果将IFN浓度增加到1000 U/ml，病毒增殖的抑制会更快。在去除IFN后的48小时内，这种抗病毒状态逐渐消失。测试了几种已知作用于细胞膜或细胞骨架的化合物对抗病毒状态建立和逆转的影响。发现它们均未对这两个参数产生显著影响。相反，将HeLa细胞置于缺乏NaCl的培养基中可部分逆转IFN处理诱导的对病毒增殖的阻断。用IFN处理后再置于低渗培养基中的细胞在感染脑心肌炎病毒后合成了病毒蛋白，尽管与未用IFN处理的细胞相比水平有所降低。
