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从总状毛霉中纯化一种可溶性和一种细胞壁结合形式的β-葡萄糖苷酶。

Purification of a soluble and a wall-bound form of beta-glucosidase from Mucor racemosus.

作者信息

Borgia P I, Mehnert D W

出版信息

J Bacteriol. 1982 Feb;149(2):515-22. doi: 10.1128/jb.149.2.515-522.1982.

DOI:10.1128/jb.149.2.515-522.1982
PMID:6173375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216536/
Abstract

beta-Glucosidase activity in crude extracts of Mucor racemosus exists in a soluble form and in a wall-bound form which sediments at 3,500 x g. The soluble form and a wall-bound form were purified to homogeneity by ammonium sulfate fractionation. DEAE-Sephadex chromatography, and SP-Sephadex chromatography. Both forms were identical in all parameters measured. Each enzyme is a glycoprotein of 91,000 daltons, with an identical amino acid composition and N-terminal amino acid of lysine; both contain about 10% carbohydrate. Both forms catalyze the hydrolysis of cellobiose and p-nitrophenyl-beta-D-glucoside with identical kinetic constants.

摘要

总状毛霉粗提物中的β-葡萄糖苷酶活性以可溶形式和在3500×g离心时沉淀的壁结合形式存在。通过硫酸铵分级分离、DEAE-葡聚糖凝胶色谱和SP-葡聚糖凝胶色谱将可溶形式和壁结合形式纯化至同质。在所有测量参数中，两种形式均相同。每种酶都是一种91,000道尔顿的糖蛋白，具有相同的氨基酸组成和赖氨酸的N端氨基酸；两者都含有约10%的碳水化合物。两种形式催化纤维二糖和对硝基苯基-β-D-葡萄糖苷的水解，动力学常数相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/6bffdabba7ed/jbacter00261-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/5c48108a1d31/jbacter00261-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/b7b02ecbd2b5/jbacter00261-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/518a13653a75/jbacter00261-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/6bffdabba7ed/jbacter00261-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/5c48108a1d31/jbacter00261-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/b7b02ecbd2b5/jbacter00261-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/518a13653a75/jbacter00261-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/900b/216536/6bffdabba7ed/jbacter00261-0116-a.jpg

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本文引用的文献

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