轴突微管的极性取向。
Polarity orientation of axonal microtubules.
作者信息
Heidemann S R, Landers J M, Hamborg M A
出版信息
J Cell Biol. 1981 Dec;91(3 Pt 1):661-5. doi: 10.1083/jcb.91.3.661.
The polarity orientation of cellular microtubules is widely regarded to be important in understanding the control of microtubule assembly and microtubule-based motility in vivo. We have used a modification of the method of Heidemann and McIntosh (Nature (Lond.). 286:517-519) to determine the polarity orientation of axonal microtubules in postganglionic sympathetic fibers of the cat. In fibers from three cats we were able to visualize the polarity of 68% of the axonal microtubules; of these, 96% showed the same polarity orientation. Our interpretation is that the rapidly growing end of all axonal microtubules is distal to the cell body. We support Kirschner's hypothesis on microtubule organizing centers. (J. Cell Biol. 86:330-334), although this interpretation raises questions about the continuity of axonal microtubules. Our results are inconsistent with a number of models for axonal transport based on force production on the surface of microtubules in which the direction of force is determined by the polarity of microtubules.
细胞微管的极性方向在理解体内微管组装控制和基于微管的运动方面被广泛认为是重要的。我们采用了对海德曼和麦金托什方法（《自然》（伦敦）。286:517 - 519）的一种改进，来确定猫节后交感神经纤维中轴突微管的极性方向。在来自三只猫的纤维中，我们能够观察到68%的轴突微管的极性；其中，96%显示出相同的极性方向。我们的解释是，所有轴突微管的快速生长端都位于远离细胞体的一端。我们支持柯施纳关于微管组织中心的假说（《细胞生物学杂志》86:330 - 334），尽管这种解释引发了关于轴突微管连续性的问题。我们的结果与一些基于微管表面产生力的轴突运输模型不一致，在这些模型中，力的方向由微管的极性决定。
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