Modrow S, Wolf H
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5703-7. doi: 10.1073/pnas.83.15.5703.
Two related but differentially expressed potential membrane proteins of Epstein-Barr virus are encoded by the same reading frame in the EcoRI D het region of the viral genome. Potential antigenic sites in the amino acid sequence of these proteins were selected by computer-aided prediction of the secondary structure and two oligopeptides corresponding to regions located in different parts of the proteins were synthesized chemically. Rabbit antisera to these peptides were used for immunoprecipitation of the native viral proteins from Epstein-Barr virus-positive cell lines from various sources. Both predicted membrane proteins could be precipitated from cell lines that had been transformed in vitro with EBV or from cell lines derived from infectious mononucleosis patients. In cell lines established from Burkitt lymphoma, only the smaller polypeptide, which lacks 138 amino acids from the amino terminus, could be identified. Using the synthetic peptides as antigens in ELISA, we detected elevated antibody titers in sera from patients with infectious mononucleosis and nasopharyngeal carcinoma.
爱泼斯坦-巴尔病毒的两种相关但表达有差异的潜在膜蛋白，由病毒基因组EcoRI D杂合区的同一阅读框编码。通过计算机辅助预测二级结构，在这些蛋白的氨基酸序列中选择潜在的抗原位点，并化学合成了与蛋白不同部位相对应的两种寡肽。用针对这些肽的兔抗血清对来自各种来源的爱泼斯坦-巴尔病毒阳性细胞系中的天然病毒蛋白进行免疫沉淀。两种预测的膜蛋白都可以从用EBV体外转化的细胞系或来自传染性单核细胞增多症患者的细胞系中沉淀出来。在从伯基特淋巴瘤建立的细胞系中，只能鉴定出较小的多肽，该多肽从氨基末端缺少138个氨基酸。使用合成肽作为ELISA中的抗原，我们检测到传染性单核细胞增多症和鼻咽癌患者血清中的抗体滴度升高。
