Roberton A M, Stanley R A
Appl Environ Microbiol. 1982 Feb;43(2):325-30. doi: 10.1128/aem.43.2.325-330.1982.
A method for isolating pig colon mucin in a soluble high-molecular-weight form, suitable for addition to bacterial growth media, is described. This preparation was utilized as a sole carbohydrate energy source by two strains of Bacteroides fragilis. The extent of degradation was compared with that of commercial pig gastric mucin by the same strains. Gas-liquid chromatographic analysis of the mucin carbohydrates and gel chromatography of the preparations were carried out before and after in vitro degradation. The mucin carbohydrates were utilized only to a very limited extent, colon mucin being more resistant to degradation than gastric mucin. Both mucins chromatographed at or near the excluded volume on Sepharose 4B, and only in the case of ATCC 25285 grown on gastric mucin was a significant degradation peak detected. If mucins are degraded in vivo by the sequential action of several bacteria, a pure culture in vitro might be expected to degrade mucins to a limited extent only. Techniques previously used to examine mucin utilization by pure cultures may have overlooked limited mucin degradation demonstrated by the methods used in this work.
本文描述了一种分离出可溶的高分子量形式猪结肠粘蛋白的方法，该方法适合添加到细菌生长培养基中。这种制剂被两株脆弱拟杆菌用作唯一的碳水化合物能量来源。将这两株菌对该制剂的降解程度与对市售猪胃粘蛋白的降解程度进行了比较。在体外降解前后，对粘蛋白碳水化合物进行了气液色谱分析，并对制剂进行了凝胶色谱分析。粘蛋白碳水化合物的利用率非常有限，结肠粘蛋白比胃粘蛋白更耐降解。两种粘蛋白在琼脂糖4B上的色谱图都在排阻体积处或其附近，只有在以胃粘蛋白为培养基生长的ATCC 25285的情况下，才检测到一个明显的降解峰。如果粘蛋白在体内被几种细菌的相继作用降解，那么体外纯培养可能只会在有限程度上降解粘蛋白。以前用于检测纯培养物对粘蛋白利用情况的技术可能忽略了本研究方法所显示的有限的粘蛋白降解。
