Ochałek T, Porwit-Bóbr Z, Radzioch D
Folia Histochem Cytochem (Krakow). 1981;19(4):189-96.
The incorporation of 3H-thymidine was used as a means to determine the stimulation of thymus and lymph node cell populations by TSTA obtained and purified from the surface of polyoma cells in a conditioned medium with T cell growth factor. The specificity of TSTA was demonstrated after the immunization of CBA mice in adoptive transfer of lymph node cells followed by challenge with polyoma cells. The binding affinity of TSTA to receptors of lymph node cells and thymocytes from CBA mice was tested by radiolabelling. Differences in binding affinity estimated on the basis of equilibrium constant (K) and maximal binding capacity (n) were found between thymus and lymph mode cells populations but not between lymph node cells populations themselves. The lack of change in the binding affinity of 125I-TSTA to receptors of lymph node cells in various experimental mice was also discussed.
利用3H-胸腺嘧啶核苷掺入法，来确定从多瘤细胞表面获取并纯化的肿瘤特异性移植抗原（TSTA），在含有T细胞生长因子的条件培养基中对胸腺和淋巴结细胞群体的刺激作用。在用多瘤细胞攻击之前，先将CBA小鼠的淋巴结细胞进行过继转移并免疫，之后证明了TSTA的特异性。通过放射性标记测试了TSTA与CBA小鼠淋巴结细胞和胸腺细胞受体的结合亲和力。根据平衡常数（K）和最大结合容量（n）估算，发现胸腺和淋巴结细胞群体之间存在结合亲和力差异，但淋巴结细胞群体自身之间没有差异。还讨论了在各种实验小鼠中125I-TSTA与淋巴结细胞受体的结合亲和力缺乏变化的情况。
