Quantitative assay of binding affinity of tumor associated antigen (TAA) to receptors in monitoring of lymphocyte populations generated in culture.

The binding affinity of 125I-TAA(tumor associated antigen) to in vitro restimulated TAA-primed lymph node cells (LNC) from CBA mice was studied by using saturation analysis. Differences between values of equilibrium constant K of antigen-lymphocyte reactions were found for in vitro TAA-stimulated TAA-primed and unprimed lymphocytes. On the other hand it has been established that TAA binding affinity increased not only as a result of TAA-specific lymphocytes restimulation but as a result of nonspecific Con A stimulation as well. The overall conclusion from the study is that saturation analysis might have been a useful tool in a preliminary monitoring of in vitro lymphocytes-antigen interactions but for the explanation of these phenomena others methods would be also used.