Ochałek T, Porwit-Bóbr Z, Radzioch D
Folia Histochem Cytochem (Krakow). 1982;20(3-4):103-8.
The binding affinity of 125I-TAA(tumor associated antigen) to in vitro restimulated TAA-primed lymph node cells (LNC) from CBA mice was studied by using saturation analysis. Differences between values of equilibrium constant K of antigen-lymphocyte reactions were found for in vitro TAA-stimulated TAA-primed and unprimed lymphocytes. On the other hand it has been established that TAA binding affinity increased not only as a result of TAA-specific lymphocytes restimulation but as a result of nonspecific Con A stimulation as well. The overall conclusion from the study is that saturation analysis might have been a useful tool in a preliminary monitoring of in vitro lymphocytes-antigen interactions but for the explanation of these phenomena others methods would be also used.
采用饱和分析法研究了¹²⁵I标记的肿瘤相关抗原（TAA）与体外再次刺激的来自CBA小鼠的经TAA致敏的淋巴结细胞（LNC）的结合亲和力。发现体外TAA刺激的经TAA致敏和未致敏的淋巴细胞的抗原-淋巴细胞反应平衡常数K值存在差异。另一方面，已经确定TAA结合亲和力不仅因TAA特异性淋巴细胞的再次刺激而增加，也因非特异性刀豆蛋白A刺激而增加。该研究的总体结论是，饱和分析可能是初步监测体外淋巴细胞-抗原相互作用的有用工具，但要解释这些现象还需使用其他方法。
