Butchko G M, Magoc T J, Aspinall R L, Nakao A
Immunol Lett. 1982 Jan;4(1):45-8. doi: 10.1016/0165-2478(82)90076-1.
A new method has been developed to detect antigen-induced histamine release in the blood of allergic rats. This in vivo model of systemic anaphylaxis has been utilized to evaluate drugs for histamine release inhibition activity. Compounds were administered by various routes at appropriate times prior to or concomitant with antigen challenge. One minute after challenge the rats were bled into tubes containing heparin and aminoguanidine. Histamine concentrations were determined by a radioenzyme technique after separation of the plasma. Experiments demonstrated that: (a) rats passively sensitized with reaginic serum exhibited elevated blood histamine upon i.v. antigen challenge in a reproducible and dose-dependent manner; (b) heat-treatment of the reaginic serum abolishes antigen-induced histamine release; and (c) antigen-induced histamine release is inhibited by disodium cromoglycate, in a dose-dependent manner and it is also inhibited by isoproterenol or isobutyl-methylxanthine.
已开发出一种新方法来检测变应性大鼠血液中抗原诱导的组胺释放。这种全身性过敏反应的体内模型已被用于评估具有组胺释放抑制活性的药物。在抗原攻击之前或同时，于适当时间通过各种途径给予化合物。攻击后一分钟，将大鼠采血至含有肝素和氨基胍的试管中。分离血浆后，通过放射酶技术测定组胺浓度。实验表明：(a) 用反应素血清被动致敏的大鼠在静脉注射抗原攻击后，血液组胺水平以可重复且剂量依赖的方式升高；(b) 反应素血清的热处理消除了抗原诱导的组胺释放；(c) 抗原诱导的组胺释放受到色甘酸二钠的抑制，呈剂量依赖性，异丙肾上腺素或异丁基甲基黄嘌呤也可抑制该释放。
