Klemm N
J Histochem Cytochem. 1982 Apr;30(4):398-400. doi: 10.1177/30.4.6174569.
A simple and rapid method is described to demonstrate catecholamine-containing neurons in the insect brain by cryostat sectioning and immersion in an ice-cold SPG (sucrose-potassium phosphate-glyoxylic acid) solution. The fluorescent yield achieved can be further intensified by heating the preparations with formaldehyde vapors. The advantages of the method as compared to the original Falck-Hillarp method are speed, simplicity, and a very distinct photostabile fluorophore. The preparations can be stored for several weeks. The drawback is a lower sensitivity than the Falck-Hillarp method provides.
本文描述了一种简单快速的方法，通过冷冻切片并将其浸入冰冷的SPG（蔗糖 - 磷酸钾 - 乙醛酸）溶液中，来显示昆虫脑中含儿茶酚胺的神经元。通过用甲醛蒸汽加热制剂，可进一步增强所获得的荧光产率。与原始的Falck - Hillarp方法相比，该方法的优点是速度快、操作简单，并且具有非常明显的光稳定荧光团。制剂可以保存数周。缺点是灵敏度低于Falck - Hillarp方法。
