Watson S J, Barchas J D
Psychopharmacol Commun. 1975;1(5):523-31.
This paper describes an adaptation of glyoxylic acid histofluorescence to cryostat sections. The technique utilizes unperfused frozen brain, cryostat sectioning, immersion in 2% glyoxylic acid solution, warm-air drying, and exposure to hot glyoxylic acid gas. Fine, well-localized catecholamine histofluorescence is produced in cells, axons, and terminals. Both the anatomical localization and pharmacological specificity of the fluorescence conform to traditional catecholamine literature. The technique has the advantage of overcoming preservation and sectioning problems associated with the Vibratome. Because unperfused brain is used, alternate sections can be prepared for a variety of anatomical demonstrations or biochemical assays.
本文描述了将乙醛酸组织荧光法应用于低温恒温器切片的方法。该技术利用未经灌注的冷冻脑、低温恒温器切片、浸入2%乙醛酸溶液、热风干燥以及暴露于热乙醛酸气体中。在细胞、轴突和终末中产生精细、定位良好的儿茶酚胺组织荧光。荧光的解剖学定位和药理学特异性均符合传统的儿茶酚胺文献。该技术的优点是克服了与振动切片机相关的保存和切片问题。由于使用的是未经灌注的脑，因此可以制备交替切片用于各种解剖学示教或生化分析。
