从尿液中快速半定量分离β2微球蛋白。
Rapid semi-quantitative isolation of beta-2-microglobulin from urine.
作者信息
Curry R, Thöen J, Messner R P
出版信息
J Immunol Methods. 1981;47(3):365-73. doi: 10.1016/0022-1759(81)90293-3.
PMID:6174634
Abstract
A rapid semi-quantitative purification of beta 2-microglobulin from transplant urine relies on a batch absorption using hydroxylapatite-cellulose. Stepwise elution is followed by Sephadex G-150 gel permeation chromatography. Using this methodology, it is possible to process quickly and conveniently many liters of urine at bench scale, with yields exceeding 95% and overall purity greater than 97% beta 2-microglobulin.
摘要
一种从移植尿液中快速半定量纯化β2-微球蛋白的方法依赖于使用羟基磷灰石-纤维素的批量吸附。随后通过Sephadex G-150凝胶渗透色谱进行分步洗脱。使用这种方法，可以在实验室规模快速方便地处理许多升尿液，产率超过95%，β2-微球蛋白的总体纯度大于97%。
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