Semple T U, Moore G E, Morgan R T, Woods L K, Quinn L A
J Natl Cancer Inst. 1982 Mar;68(3):365-80.
Of 23 cell lines representing 8 patients with malignant melanoma, one cell line from each patient has been extensively characterized by means of phase morphology, ultrastructural morphology, biochemical markers, steroid receptor protein analysis, and steroid hormone production. Extensive cytogenetic characterization was compiled from seven of the eight cell lines. The melanocytic derivation of the tumor cell lines was supported by the detection of the catecholamines epinephrine, norepinephrine, and dopamine. In addition, well-defined melanosomes were present in six of the seven lines whose ultrastructure were examined morphologically. None of the lines produced alpha-fetoprotein chorionic gonadotropin, or carcinoembryonic antigen in detectable amounts. The individuality of the cell lines was confirmed by phenotypic patterns of isozymes and by karyology.
在代表8例恶性黑色素瘤患者的23个细胞系中，每个患者的一个细胞系已通过相形态学、超微结构形态学、生化标志物、类固醇受体蛋白分析和类固醇激素产生进行了广泛表征。从8个细胞系中的7个细胞系收集了广泛的细胞遗传学特征。肿瘤细胞系的黑素细胞来源得到了儿茶酚胺肾上腺素、去甲肾上腺素和多巴胺检测结果的支持。此外，在7个经形态学检查超微结构的细胞系中，有6个存在明确的黑素小体。这些细胞系均未产生可检测量的甲胎蛋白、绒毛膜促性腺激素或癌胚抗原。细胞系的个体性通过同工酶的表型模式和核型分析得到证实。
