Kovarík J, Svejda J, Bucek J, Rejthar A, Král B, Ninger E, Zemanová D, Lauerová L
Neoplasma. 1978;25(6):701-12.
A new human cell line of malignant melanoma (MJM) was established with the use of the in vitro fragment technique. It has been maintained over 34 months of continuous cultivation. Three types of cells can be recognized by light microscope. The epitheloid elements predominate, less frequent are fibroblastoid and giant multinuclear cells. The pigment production is not macroscopically visible. Over 60 per cent of analyzed metabphases showed hyperdiploid number of chromosomes, the rest was mostly tetra and hexaploid. No marker chromosomes were detected. The growth studies indicate the MJM cells have 63-hr doubling time. Cytochemistry revealed positive pigment or propigment granules in 36 per cent of cells. Ultrastructural studies did not detect melanin granules but some particles resembling atypical premelanosomes and melanosomes were recognized in some sections.
利用体外片段技术建立了一种新的恶性黑色素瘤人类细胞系（MJM）。它已经连续培养了34个月以上。通过光学显微镜可识别出三种类型的细胞。上皮样细胞成分占主导，成纤维细胞样细胞和巨大多核细胞较少见。肉眼看不到色素产生。超过60%的分析中期细胞显示染色体数目超二倍体，其余大多为四倍体和六倍体。未检测到标记染色体。生长研究表明MJM细胞的倍增时间为63小时。细胞化学显示36%的细胞中有阳性色素或色素原颗粒。超微结构研究未检测到黑色素颗粒，但在一些切片中识别出了一些类似非典型前黑素小体和黑素小体的颗粒。
