感染鼠冠状病毒JHM的细胞中病毒RNA的特征分析

Characterisation of viral RNA in cells infected with the murine coronavirus JHM.

作者信息

Wege H, Siddell S, Sturm M, ter Meulen V

出版信息

Adv Exp Med Biol. 1981;142:91-101. doi: 10.1007/978-1-4757-0456-3_7.

Abstract

The murine coronavirus JHM induces in Sac(-) cells seven major and two minor RNA species. These RNAs are polyadenylated and single stranded. Their sizes were estimated by electrophoresis in agarose gels containing methylmercury hydroxide. The mol. wts. for the major species are 6.67 x 10(6) for RNA of genome size, 3.42 x 10(6) for RNA 2, 2.76 x 10(6) for RNA 3, 1.35 x 10(6) for RNA 4, 1.19 x 10(6) for RNA 5, 0.93 x 10(6) for RNA 6 and 0.62 x 10(6) for RNA 7. The minor species have a size of 4.7 x 10(6) (RNA a) and 1.5 x 10(6) (RNA b). No essential difference in the number and proportion of each RNA species was found between total cytoplasmic RNA, polyadenylated cytoplasmic RNA and RNA extracted from pelleted polysomes, nor was any difference found during the infection cycle. The major RNA species are likely to be subgenomic mRNAs.

摘要

鼠冠状病毒JHM在Sac(-)细胞中诱导产生七种主要RNA种类和两种次要RNA种类。这些RNA是多聚腺苷酸化的单链RNA。通过在含有氢氧化甲基汞的琼脂糖凝胶中进行电泳来估计它们的大小。主要种类的分子量分别为：基因组大小的RNA为6.67×10(6)，RNA 2为3.42×10(6)，RNA 3为2.76×10(6)，RNA 4为1.35×10(6)，RNA 5为1.19×10(6)，RNA 6为0.93×10(6)，RNA 7为0.62×10(6)。次要种类的大小分别为4.7×10(6)（RNA a）和1.5×10(6)（RNA b）。在总细胞质RNA、多聚腺苷酸化细胞质RNA和从沉淀的多核糖体中提取的RNA之间，未发现每种RNA种类的数量和比例有本质差异，在感染周期中也未发现任何差异。主要RNA种类可能是亚基因组mRNA。