Wege H, Müller A, ter Meulen V
J Gen Virol. 1978 Nov;41(2):217-27. doi: 10.1099/0022-1317-41-2-217.
Genomic RNA extracted from the purified murine coronavirus JHM sediments between 52S and 54S in aqueous sucrose gradients. The RNA is single-stranded and has an apparent mol. wt. of 5.4 to 6.5 X 10(6), as determined by electrophoresis in polyacrylamide agarose gels of different concentrations. The presence of polyadenylate sequences in the RNA is demonstrated by binding to oligo-)dT) cellulose and digestion with ribonucleases A and T1. The purified RNA does not dissociate into subunits at high temperatures or in high concentrations of DMSO and is infectious.
从纯化的鼠冠状病毒JHM中提取的基因组RNA在蔗糖水溶液梯度中沉降于52S和54S之间。该RNA为单链，通过在不同浓度的聚丙烯酰胺琼脂糖凝胶中电泳测定，其表观分子量为5.4至6.5×10⁶。通过与寡聚（dT）纤维素结合并用核糖核酸酶A和T1消化，证明RNA中存在聚腺苷酸序列。纯化的RNA在高温或高浓度二甲基亚砜中不会解离成亚基，并且具有感染性。
