小鼠肝炎病毒的核糖核酸
RNA of mouse hepatitis virus.
作者信息
Lai M M, Stohlman S A
出版信息
J Virol. 1978 May;26(2):236-42. doi: 10.1128/JVI.26.2.236-242.1978.
Abstract
The RNA of mouse hepatitis virus, a coronavirus, was isolated from the virus released early in the infection and analyzed by sucrose gradient sedimentation and electrophoresis. It was found to consist of a piece of single-stranded RNA of about 60S. Its molecular weight was estimated to be 5.4 X 10(6) by electrophoresis in methylmercury-agarose gels. At least one third of the RNA contained polyadenylated sequences. It is, therefore, probably positive stranded. The virus harvested late in the infection contained, in addition to 60S, some 30 to 50S RNA that are possibly degradation products of the 60S RNA. No difference in the electrophoretic behavior could be detected between the RNA isolated from a pathogenic (JHM) and a nonpathogenic (A59) strain.
摘要
从小鼠肝炎病毒（一种冠状病毒）感染早期释放的病毒中分离出其RNA，并通过蔗糖梯度沉降和电泳进行分析。发现它由一段约60S的单链RNA组成。通过在甲基汞 - 琼脂糖凝胶中电泳，其分子量估计为5.4×10⁶。至少三分之一的RNA含有聚腺苷酸化序列。因此，它可能是正链的。感染后期收获的病毒除了含有60S RNA外，还含有一些30至50S的RNA，这些可能是60S RNA的降解产物。从致病性（JHM）和非致病性（A59）毒株分离出的RNA在电泳行为上未检测到差异。
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