一种新型抗炎剂筛选方法。用电免疫分析法测定大鼠血清急性期α2-巨球蛋白水平。

A new screen for anti-inflammatory agents. Estimation of rat serum acute phase alpha2-macroglobulin levels using an electroimmunoassay.

作者信息

Baldo B A, Chow S C, Euers C

出版信息

Agents Actions. 1981 Nov;11(5):482-9. doi: 10.1007/BF02004710.

DOI:10.1007/BF02004710

Abstract

Induction of alpha2-macroglobulin acute phase protein in rats by the injection of turpentine revealed strain and sex differences in the amounts of this protein detected in sera by rocket immunoelectrophoresis. Studies in vivo with a wide variety of drugs showed that non-steroidal anti-inflammatory drugs (NSAID) such as aspirin, indomethacin and BW 755C as well as colchicine, clofibrate and clozic, inhibited serum concentrations of the acute phase protein 48 h after turpentine injection. The procedure used offers a new and convenient screen for assessing NSAIDs and the method may be of value as a screen for new clozic-like compounds with anti-rheumatic activity. The possibility that the NSAIDs are inhibiting a humoral factor initiated by the inflammatory stimulus and that the humoral factor is a metabolite of arachidonic acid is discussed.

摘要

通过注射松节油诱导大鼠产生α2-巨球蛋白急性期蛋白，结果显示，采用火箭免疫电泳法检测血清中该蛋白的含量时存在品系和性别差异。对多种药物进行的体内研究表明，阿司匹林、消炎痛和BW 755C等非甾体抗炎药（NSAID）以及秋水仙碱、氯贝丁酯和氯氮平，在注射松节油48小时后可抑制急性期蛋白的血清浓度。所采用的方法为评估NSAID提供了一种新的便捷筛选方法，该方法作为筛选具有抗风湿活性的新型氯氮平样化合物的手段可能具有价值。文中讨论了NSAID抑制由炎症刺激引发的体液因子以及该体液因子是花生四烯酸代谢产物的可能性。