Shimizu Y, Oshima K, Nakano Y, Fujita M, Taguchi T
Biken J. 1981 Sep;24(3):95-102.
Pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) levels were measured in human sera by a modification of Laurell's electroimmunoassay using rabbit anti-alpha 2-PAG serum. Sera were obtained from healthy controls (32 males and 46 females), patients with benign breast diseases (55 cases), and patients with breast (82 cases), gastric (89 cases), or colorectal (22 cases) cancers. In healthy controls, the mean alpha 2-PAG value for females was higher than that for males (p less than 0.05), so alpha 2-PAG values for males and females were considered separately in this study. Serum alpha 2-PAG levels in patients with benign breast tumors were almost the same as those of controls. In patients with primary breast and gastric cancer, alpha 2-PAG levels were higher than those of controls (p less than 0.005) and tended to increase with progress of the disease. Raised alpha 2-PAG levels decreased in these patients after curative surgery. These results show that serum alpha 2-PAG is useful as a marker in both the initial diagnosis and follow-up of breast and gastric cancer. The reliability of alpha 2-PAG as a tumor-associated marker was reinforced by comparison of the positive rates of the three parameters alpha 2-PAG, carcinoembryonic antigen (CEA), and immunosuppressive acidic protein (IAP) in patients with breast and gastric cancer.
采用改良的劳雷尔电免疫分析法，使用兔抗α2-妊娠相关糖蛋白（α2-PAG）血清，测定人血清中的α2-PAG水平。血清取自健康对照者（32名男性和46名女性）、患有良性乳腺疾病的患者（55例）以及患有乳腺癌（82例）、胃癌（89例）或结直肠癌（22例）的患者。在健康对照者中，女性的α2-PAG平均水平高于男性（p<0.05），因此本研究分别考虑男性和女性的α2-PAG值。良性乳腺肿瘤患者的血清α2-PAG水平与对照者几乎相同。原发性乳腺癌和胃癌患者的α2-PAG水平高于对照者（p<0.005），且往往随疾病进展而升高。这些患者在根治性手术后，升高的α2-PAG水平下降。这些结果表明，血清α2-PAG在乳腺癌和胃癌的初始诊断及随访中均可用作标志物。通过比较乳腺癌和胃癌患者中α2-PAG、癌胚抗原（CEA）和免疫抑制酸性蛋白（IAP）这三个参数的阳性率，增强了α2-PAG作为肿瘤相关标志物的可靠性。
