Starr S E, Garrabrant T
Clin Exp Immunol. 1981 Dec;46(3):484-92.
The ability of human peripheral blood mononuclear (MN) cells to lyse uninfected and cytomegalovirus (CMV) infected human fibroblasts was determined in a 51Cr-release assay. Maximal release was obtained with 6-day infected fibroblasts incubated with MN cells for 24 hr. A linear relationship existed between E/T ratios of 12.5:1 to 100:1 and lysis of CMV-infected targets. Donor immune status had no effect on the magnitude of killing of infected or uninfected targets. Killing was mediated by non-B, predominantly non-T, Fc receptor-bearing cells. Preincubation of effector cells with interferon enhanced killing of both CMV-infected and uninfected fibroblasts, but infected targets were more effectively killed. These results indicated a possible role for natural killer cells in recovery from CMV infection.
采用铬-51释放试验测定人外周血单个核(MN)细胞对未感染及巨细胞病毒(CMV)感染的人成纤维细胞的裂解能力。将6天感染的成纤维细胞与MN细胞孵育24小时可获得最大释放量。效应细胞与靶细胞(E/T)比例在12.5:1至100:1之间时,与CMV感染靶细胞的裂解呈线性关系。供体免疫状态对感染或未感染靶细胞的杀伤程度无影响。杀伤由非B细胞、主要是非T细胞且带有Fc受体的细胞介导。效应细胞与干扰素预孵育可增强对CMV感染及未感染成纤维细胞的杀伤,但对感染靶细胞的杀伤更有效。这些结果表明自然杀伤细胞在从CMV感染中恢复过程中可能发挥作用。