用巨细胞病毒抗原刺激成人人类白细胞培养物中的淋巴细胞增殖及干扰素产生。
Lymphocyte blastogenesis and interferon production in adult human leukocyte cultures stimulated with cytomegalovirus antigens.
作者信息
Starr S E, Dalton B, Garrabrant T, Paucker K, Plotkin S A
出版信息
Infect Immun. 1980 Oct;30(1):17-22. doi: 10.1128/iai.30.1.17-22.1980.
Abstract
Lymphocyte blastogenesis and interferon production were measured in adult human leukocyte cultures stimulated with purified or crude cytomegalovirus antigens. Leukocytes from seropositive adults underwent blastogenesis when stimulated with purified or crude Towne strain antigen, whereas neither antigen stimulated blastogenesis in cultures from seronegative donors. The concentrations of antigens yielding maximal blastogenesis varied among the individuals tested. When cultures from seropositive individuals were stimulated with antigens prepared from three different CMV strains--AD-169, Towne, and Davis--comparable levels of blastogenesis were detected. Type 1 interferon was detected in supernatants of cultures stimulated with crude antigens regardless of the immune status of the donor. In contrast, when purified antigen was used as the stimulant, only cultures obtained from seropositive individuals produced detectable levels of interferon, which appeared to be predominantly type 2 or immune interferon.
摘要
在使用纯化的或粗制的巨细胞病毒抗原刺激的成人白细胞培养物中，检测淋巴细胞增殖和干扰素产生情况。来自血清反应阳性成年人的白细胞在用纯化的或粗制的汤氏株抗原刺激时会发生增殖，而两种抗原均未刺激血清反应阴性供体培养物中的细胞增殖。产生最大增殖反应的抗原浓度在受试个体之间有所不同。当用由三种不同巨细胞病毒株——AD - 169、汤氏株和戴维斯株制备的抗原刺激血清反应阳性个体的培养物时，检测到了相当水平的细胞增殖。无论供体的免疫状态如何，在用粗制抗原刺激的培养物上清液中均检测到了1型干扰素。相比之下，当使用纯化抗原作为刺激物时，只有从血清反应阳性个体获得的培养物产生了可检测水平的干扰素，这些干扰素似乎主要是2型或免疫干扰素。
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Plaque assay of cytomegalovirus strains of human origin.人源巨细胞病毒毒株的空斑试验
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