Lenhard V, Diehm H, Römer W, Rauterberg E W, Kownatzki E, Roelcke D
Immunobiology. 1982 Feb;160(5):382-91. doi: 10.1016/S0171-2985(82)80002-8.
A case of a 35-year-old female with multiple sclerosis is reported who developed without apparent prior sensitization a lymphocytotoxic antibody with anti-HLA-B8 specificity. The antibody persisted for several years with the same titer. The cytotoxic activity of the patient's serum was contained within the IgM fraction. The antibody reacted optimally at low temperature, exclusively against lymphocytes homozygous for HLA-B8. In B8-heterozygous cells, cytotoxic reactions were obtained only following enzymatic pretreatment. The antibody's binding avidity was weak; for its complete absorption, many times more B8-positive lymphocytes or platelets were needed than for a "normal" anti-B8 antibody of the same titer. In HLA redistribution and blocking experiments, it was demonstrated that the antigenic determinant recognized by this antibody is carried by the B8 molecule. It is unclear whether "spontaneously" occurring cold-reactive IgM antibodies with HLA specificity are induced by viral agents or whether they reflect "spontaneous" clonal lymphocyte proliferation.
报道了一例35岁的多发性硬化症女性患者，该患者在无明显先前致敏的情况下产生了具有抗HLA - B8特异性的淋巴细胞毒性抗体。该抗体以相同滴度持续存在数年。患者血清的细胞毒性活性存在于IgM组分中。该抗体在低温下反应最佳，仅针对HLA - B8纯合的淋巴细胞。在B8杂合细胞中，仅在酶预处理后才获得细胞毒性反应。该抗体的结合亲和力较弱；为了完全吸收，所需的B8阳性淋巴细胞或血小板比相同滴度的“正常”抗B8抗体多许多倍。在HLA再分布和阻断实验中，证明该抗体识别的抗原决定簇由B8分子携带。尚不清楚具有HLA特异性的“自发”产生的冷反应性IgM抗体是由病毒因子诱导的，还是它们反映了“自发”的克隆淋巴细胞增殖。
