Kasahara T, Harada H, Shioiri-Nakano K, Wakasugi H, Imai M, Mayumi M, Sano T, Sugiura A
Immunology. 1982 Apr;45(4):687-95.
In the previous paper we reported that human natural killer (NK) cell activity was augmented greatly by preincubation with Staphylococcus aureus Cowan I bacteria (SpA CoI) or its Protein A. We examined here whether the augmentation with these stimulants is ascribable to the direct activation of NK cells or mediated by some soluble factors produced by the stimulants. It was found that a significant amount of interferon (IFN) was produced by the SpA CoI-stimulation but not by the Protein A-stimulation, although the latter usually induced augmentation of NK-cell activity not less than SpA CoI-stimulation. IFN produced by SpA CoI was considered to belong to alpha-type IFN, because it was stable at pH 2.0 and could be neutralized effectively by anti-IFN alpha antibody. Kinetics of NK-cell activation by SpA CoI (but not by Protein A) were very similar to those by IFN alpha. Furthermore, augmentation of NK-cell activity with SpA CoI-stimulated supernatant was inhibited almost completely by diluted anti-IFN alpha antibody, whereas augmentation with Protein A-stimulated supernatant could not be abolished by the same treatment. It was, therefore, suggested that augmentation of NK-cell activity with SpA CoI might be ascribable in most part to the IFN induced, whereas Protein A can stimulated NK or T cells directly or soluble factors other than IFN might work as well.
在之前的论文中，我们报道了人自然杀伤（NK）细胞活性通过与金黄色葡萄球菌Cowan I菌（SpA CoI）或其蛋白A预孵育而大幅增强。我们在此研究了这些刺激剂所引起的活性增强是归因于NK细胞的直接激活，还是由刺激剂产生的某些可溶性因子介导。结果发现，SpA CoI刺激可产生大量干扰素（IFN），而蛋白A刺激则不会产生，尽管后者通常诱导的NK细胞活性增强程度不低于SpA CoI刺激。SpA CoI产生的IFN被认为属于α型IFN，因为它在pH 2.0时稳定，并且能被抗IFNα抗体有效中和。SpA CoI（而非蛋白A）激活NK细胞的动力学与IFNα激活NK细胞的动力学非常相似。此外，用稀释的抗IFNα抗体几乎完全抑制了SpA CoI刺激的上清液对NK细胞活性的增强作用，而相同处理并不能消除蛋白A刺激的上清液对NK细胞活性的增强作用。因此，提示SpA CoI增强NK细胞活性在很大程度上可能归因于诱导产生的IFN，而蛋白A可直接刺激NK或T细胞，或者可能有IFN以外的可溶性因子起作用。
