Kasahara T, Harada H, Shioiri-Nakano K, Imai M, Sano T
Immunology. 1981 Feb;42(2):175-83.
Cytotoxic activity of human lymphocytes against the myeloid cell line K-562 was augmented greatly by 24-h incubation with Staphylococcus aureus Cowan I bacteria (SpA CoI) and its protein A. This effect was not observed when these stimulants were added after preincubation, suggesting that this activity was different from so-called lectin-induced cellular cytotoxicity. Potentiation required at least 12 to 18 h incubation of lymphocytes with these stimulants. Macrophage depletion did not affect the potentiation by protein A or SpA coI, although the potentiation by poly I:C or OK-432, an immunopotentiator of Streptococcus pyogenes was completely reduced. Further cell separation procedures revealed that neither T cells nor FcR- cells, which showed little natural killer (NK) activity, were enhanced by protein A or SpA coI. On the other hand, (a) null cells which were obtained from nylon column (NC)-passed fraction by depleting T cells and surface membrane Ig-positive cells, and (b) FcR+ E- cells which were obtained from NC-passed fraction by depleting FcR- cells and T cells, showed marked NK activity by themselves and were further augmented by these stimulants. FcR+ E+ cells failed to show NK activity even if they were stimulated by these stimulants. Thus, it was found that protein A and SpA CoI, as well as human interferon, could stimulate NC-non-adherent, FcR+, E- NK cells and potentiate markedly their NK activity.
人淋巴细胞对髓系细胞系K - 562的细胞毒性活性，在与金黄色葡萄球菌Cowan I菌（SpA CoI）及其蛋白A孵育24小时后显著增强。当在预孵育后添加这些刺激物时未观察到这种效应，这表明这种活性不同于所谓的凝集素诱导的细胞毒性。淋巴细胞与这些刺激物孵育至少需要12至18小时才能实现增强作用。巨噬细胞耗竭并不影响蛋白A或SpA CoI的增强作用，尽管聚肌胞苷酸（poly I:C）或化脓性链球菌免疫增强剂OK - 432的增强作用完全被消除。进一步的细胞分离程序表明，蛋白A或SpA CoI均未增强T细胞或几乎没有自然杀伤（NK）活性的FcR⁻细胞。另一方面，（a）通过去除T细胞和表面膜免疫球蛋白阳性细胞从尼龙柱（NC）通过的部分获得的空细胞，以及（b）通过去除FcR⁻细胞和T细胞从NC通过的部分获得的FcR⁺E⁻细胞，自身具有显著的NK活性，并被这些刺激物进一步增强。即使受到这些刺激物刺激，FcR⁺E⁺细胞也未表现出NK活性。因此，发现蛋白A和SpA CoI以及人干扰素可以刺激NC非黏附、FcR⁺、E⁻NK细胞，并显著增强它们的NK活性。
