Scacciati de Cerezo J M, Bueno M P, Skowroński B, Cerezo A S
Am J Reprod Immunol (1980). 1982 Oct;2(5):246-9. doi: 10.1111/j.1600-0897.1982.tb00176.x.
A semiquantitative immunohistochemical technique for the detection of N-acetyl alpha-D-neuraminic acid, N-acetyl beta-D-glucosamine and its beta-(1 leads to 4)-linked internal chains, alpha-D-glucopyranosyl and alpha-D-mannopyranosyl and its alpha-(1 leads to 2)-linked internal chains and sterically related, nonreducing, end-chain residues of oligosaccharide chains of glycoproteins or glycolipids on the surface of membranes was developed using Con A and wheat germ lectins. When this method was applied to the localization of carbohydrate receptors on the membrane of the normal human spermatozoa, it was found that the Con A and wheat germ lectin receptors were mainly located in the equatorial and post nuclear cap with few receptors located in the acrosome and neck. None of them were found in the intermediate segment plus tail. Con A receptors were alpha-D-mannopyranosyl end-chain residues and wheat germ lectin receptors were N-acetyl beta-D-glucosamine (1 leads to 4)-linked internal chains. These groups occur together in the oligomannosidic type of N-glycosidic-linked oligosaccharide chains of glycoproteins and so the use of both lectins on desialycated membranes or on those which contain nonclustered N-acetyl neuraminic acid residues may be of help to localize this type of glycoprotein oligosaccharide chains. Con A receptors were not removed after proteases digestion, suggesting the possibility that they are part of intrinsic spermatozoal antigens.
利用伴刀豆球蛋白A（Con A）和麦胚凝集素开发了一种半定量免疫组织化学技术，用于检测膜表面糖蛋白或糖脂寡糖链的N-乙酰-α-D-神经氨酸、N-乙酰-β-D-葡糖胺及其β-（1→4）连接的内部链、α-D-吡喃葡萄糖基和α-D-吡喃甘露糖基及其α-（1→2）连接的内部链以及空间相关的、非还原性的、末端链残基。当将该方法应用于正常人精子膜上碳水化合物受体的定位时，发现Con A和麦胚凝集素受体主要位于赤道区和核后帽，在顶体和颈部的受体较少。在中段加尾部未发现这些受体。Con A受体是α-D-吡喃甘露糖基末端链残基，麦胚凝集素受体是N-乙酰-β-D-葡糖胺（1→4）连接的内部链。这些基团共同存在于糖蛋白的寡甘露糖型N-糖苷连接寡糖链中，因此在去唾液酸化膜或含有非聚集N-乙酰神经氨酸残基的膜上同时使用这两种凝集素可能有助于定位这种类型的糖蛋白寡糖链。蛋白酶消化后Con A受体未被去除，提示它们可能是精子固有抗原的一部分。
