Hinman C L, Rauch H C, Pfeifer R F
Life Sci. 1982 Mar 22;30(12):989-93. doi: 10.1016/0024-3205(82)90516-1.
Two preparations of myelin basic protein (MBP) were derived from an acid excretion of chloroform-methanol defatted bovine spinal cord. The first was purified by ion-exchange chromatography using guanidine-HCl; the second, by high performance liquid chromatography (HPLC) using a triethylamine eluant. Both methods of preparation yield MBP which is identical on acid-urea polyacrylamide gel electrophoresis and which has identical encephalitogenic potency. Because of the greater time-efficiency of the HPLC system with no deleterious side effects due to buffer contamination, this latter method can be recommended for MBP purification.
两种髓鞘碱性蛋白（MBP）制剂源自氯仿-甲醇脱脂牛脊髓的酸提取物。第一种通过使用盐酸胍的离子交换色谱法纯化；第二种通过使用三乙胺洗脱液的高效液相色谱法（HPLC）纯化。两种制备方法得到的MBP在酸性尿素聚丙烯酰胺凝胶电泳上是相同的，并且具有相同的致脑炎效力。由于HPLC系统具有更高的时间效率且不存在缓冲液污染带来的有害副作用，因此可以推荐后一种方法用于MBP的纯化。
