在豚鼠身上测试的牛少突胶质细胞纯化制剂的致脑炎性特性。

Encephalitogenic properties of purified preparations of bovine oligodendrocytes tested in guinea pigs.

作者信息

Raine C S, Traugott U, Iqbal K, Snyder D S, Cohen S R, Farooq M, Norton W T

出版信息

Brain Res. 1978 Feb 17;142(1):85-96. doi: 10.1016/0006-8993(78)90178-6.

Abstract

The present study has investigated central nervous system disease in guinea pigs inoculated with emulsions containing purified preparations of bovine oligodendroglia and their fractions isolated with or without trypsinization, whole bovine white matter or myelin basic protein (MBP). The MBP content of the oligodendroglial fractions was determined by radioimmunoassay. It was found that oligodendroglia prepared from trypsinized fresh brain contained minute amounts of MBP and did not induce disease. The corresponding cell fraction from non-trypsinized frozen brain was rich in MBP and induced disease. Bovine white matter and MBP induced typical experimental allergic encephalomyelitis (EAE). The structural preservation of the non-encephalitogenic trypsinized MBP-poor cells was very good and that of the encephalitogenic MBP-rich non-trypsinized cells very poor. It has been concluded that the encephalitogenicity observed was due to MBP, rather than to a specific oligodendroglial antigen.

摘要

本研究调查了接种含有纯化牛少突胶质细胞制剂及其经胰蛋白酶处理或未经处理分离的组分、全牛白质或髓鞘碱性蛋白（MBP）的乳剂的豚鼠中枢神经系统疾病。通过放射免疫测定法测定少突胶质细胞组分中的MBP含量。结果发现，从经胰蛋白酶处理的新鲜脑中制备的少突胶质细胞含有微量的MBP，且不会诱发疾病。来自未经胰蛋白酶处理的冷冻脑的相应细胞组分富含MBP并诱发疾病。牛白质和MBP诱发典型的实验性变态反应性脑脊髓炎（EAE）。无致脑炎作用的经胰蛋白酶处理的低MBP细胞的结构保存非常好，而有致脑炎作用的富含MBP的未经胰蛋白酶处理的细胞的结构保存非常差。得出的结论是，观察到的致脑炎作用是由于MBP，而不是由于特定的少突胶质细胞抗原。