Krivtsova M A, Moroshkina E B, Glibin E N, Frisman E V
Mol Biol (Mosk). 1982 Jan-Feb;16(1):149-55.
DNA-complexes with actinomine and its analogues containing omega-dialkylaminoalkyl groups at 1,9 positions of the phenoxazone moiety were studied by technique of spectrophotometry, viscometry and flow birefringence. In the process of spectrophotometry titration two groups of spectra corresponding to different DNA--ligand ratio in a complex were observed. According to the experimental data the investigated compounds are bounded to DNA by means of intercalation and external binding. There within a region of low degrees of binding the intercalation type of the ligand--DNA interaction prevails. In virtue of the spectrophotometry data the intercalation binding share was calculated. The intrinsic viscosity of a complex increases in the case of ligand intercalation and does not change as it joins to the DNA double-helix from outside. Optical anisotropy of DNA molecule increases linearly irrespective of the way of ligand binding. Data on the flow birefringence permits to conclude that under external binding the angle between the normal to the ligand chromophore plane and the axis of DNA double-helix is about zero. During ligand intercalation the equilibrium rigidity of DNA molecules increases.
利用分光光度法、粘度测定法和流动双折射技术研究了与放线菌素及其在吩恶嗪部分1,9位含有ω-二烷基氨基烷基的类似物形成的DNA复合物。在分光光度滴定过程中，观察到两组对应于复合物中不同DNA-配体比例的光谱。根据实验数据，所研究的化合物通过嵌入和外部结合与DNA结合。在低结合度区域内，配体- DNA相互作用的嵌入类型占主导。根据分光光度数据计算了嵌入结合份额。在配体嵌入的情况下，复合物的特性粘度增加，而当它从外部连接到DNA双螺旋时则不变。无论配体结合方式如何，DNA分子的光学各向异性都呈线性增加。流动双折射数据表明，在外部结合时，配体发色团平面的法线与DNA双螺旋轴之间的夹角约为零。在配体嵌入过程中，DNA分子的平衡刚性增加。
