Braathen L R, Thorsby E
Scand J Immunol. 1982 Jan;15(1):55-61. doi: 10.1111/j.1365-3083.1982.tb00621.x.
Epidermal cell suspensions were produced containing 3-6% HLA-DR-positive Langerhans cells. By using mixtures of epidermal cells and allogeneic T lymphocytes from donors sensitized to purified derivative of tuberculin (PPD) and herpes simplex virus (HSV), we could show that a proliferative in vitro response to these antigens, similar in strength to that seen in autologous combination of macrophages or epidermal cells and T cells, could be obtained provided the epidermal cells shared both HLA-D/DR determinants with the T-cell donor. No antigen-specific response qas obtained in fully HLA-D/DR-disparate combinations. These results, together with previously reported data using other antigen-presenting cells, further emphasize that one minimal requirement for antigen-specific activation of T cells is co-recognition of products of the self HLA-D region.
制备了含有3%-6% HLA-DR阳性朗格汉斯细胞的表皮细胞悬液。通过使用来自对结核菌素纯化衍生物（PPD）和单纯疱疹病毒（HSV）致敏的供体的表皮细胞与同种异体T淋巴细胞的混合物，我们能够证明，只要表皮细胞与T细胞供体共享HLA-D/DR决定簇，就可以获得对这些抗原的体外增殖反应，其强度与巨噬细胞或表皮细胞与T细胞的自体组合中所见的反应相似。在完全HLA-D/DR不匹配的组合中未获得抗原特异性反应。这些结果，连同先前使用其他抗原呈递细胞报道的数据，进一步强调了T细胞抗原特异性激活的一个最低要求是对自身HLA-D区域产物的共同识别。
