Berle E J, Thorsby E
Scand J Immunol. 1982 Dec;16(6):543-7. doi: 10.1111/j.1365-3083.1982.tb00757.x.
Antigen-primed T-cell blasts may be separated from alloreactive T cells on Percoll gradients. By means of this method, HLA restriction of antigen-specific proliferative T-cell responses may be studied, using allogeneic antigen-presenting cells carrying foreign D/DR antigens. The reported data confirm that the D/DR molecules as such are major restriction elements in the T-cell response to herpes simplex virus (HSV) and purified protein derivative (PPD). However, evidence is presented that other Class II HLA molecules, the MT molecules, may also function as restriction elements for the HSV response.
经抗原致敏的T细胞母细胞可通过Percoll梯度从同种异体反应性T细胞中分离出来。通过这种方法，可以利用携带外来D/DR抗原的同种异体抗原呈递细胞来研究抗原特异性增殖性T细胞反应的HLA限制性。报告的数据证实，D/DR分子本身是T细胞对单纯疱疹病毒（HSV）和纯化蛋白衍生物（PPD）反应中的主要限制性元件。然而，有证据表明，其他II类HLA分子，即MT分子，也可能作为HSV反应的限制性元件发挥作用。
