Berle E J, Thoresen A B, Thorsby E
Tissue Antigens. 1982 Nov;20(5):380-8. doi: 10.1111/j.1399-0039.1982.tb02254.x.
Antigen-specific T cell blasts may be separated from alloreactive cells on Percoll gradients. The absence of alloreactivity allowed us to study HLA-D/DR restriction of proliferative T cell responses towards PPD and HSV antigens using allogeneic antigen-presenting cells. An overriding impact of self HLA-D/DR determinants as restriction elements for in vivo sensitized T cells was demonstrated. Furthermore, evidence was obtained that the proliferative PPD response might be preferentially restricted by HLA-D/DR 1.
抗原特异性T细胞母细胞可通过Percoll梯度从同种反应性细胞中分离出来。同种反应性的缺失使我们能够利用同种异体抗原呈递细胞研究增殖性T细胞对结核菌素纯蛋白衍生物（PPD）和单纯疱疹病毒（HSV）抗原反应的HLA - D/DR限制性。研究证明了自身HLA - D/DR决定簇作为体内致敏T细胞限制性元件的首要影响。此外，有证据表明增殖性PPD反应可能优先受HLA - D/DR 1限制。
