Robertson S M, Frisch C F, Gulig P A, Kettman J R, Johnston K H, Hansen E J
Infect Immun. 1982 Apr;36(1):80-8. doi: 10.1128/iai.36.1.80-88.1982.
Monoclonal antibodies directed against several different Haemophilus influenzae type b outer membrane proteins with apparent molecular weights of 45,000, 39,000, and 37,000 were identified by a radioimmunoprecipitation method. Five monoclonal antibodies, including both immunoglobulin G and M isotypes, were specific for the same H. influenzae type b major outer membrane protein (39,000 molecular weight). One of these immunoglobulin G monoclonal antibodies (6A2) was shown to be directed against a cell surface-exposed antigenic determinant of the 39,000-molecular-weight protein, whereas the other monoclonal antibodies directed against this same protein were apparently specific for antigenic determinants not exposed on the H. Influenzae type b cell surface. The cell surface-exposed protein antigenic determinant recognized by monoclonal antibody 6A2 was not unique to the H. influenzae type b strain used as the source of outer membrane vesicles for generating immune spleen cells, but was found in a majority of independently isolated strains of H. influenzae type b. These data indicate that there is antigenic cross-reactivity among H. influenzae type b strains with regard to cell surface-exposed proteins.
通过放射免疫沉淀法鉴定出了针对几种不同的b型流感嗜血杆菌外膜蛋白的单克隆抗体，这些蛋白的表观分子量分别为45,000、39,000和37,000。五种单克隆抗体，包括免疫球蛋白G和M同种型，对同一b型流感嗜血杆菌主要外膜蛋白（分子量39,000）具有特异性。其中一种免疫球蛋白G单克隆抗体（6A2）被证明针对的是分子量为39,000的蛋白的细胞表面暴露抗原决定簇，而针对同一蛋白的其他单克隆抗体显然针对的是b型流感嗜血杆菌细胞表面未暴露的抗原决定簇。单克隆抗体6A2识别的细胞表面暴露蛋白抗原决定簇并非用作外膜囊泡来源以产生免疫脾细胞的b型流感嗜血杆菌菌株所特有，而是在大多数独立分离的b型流感嗜血杆菌菌株中都能发现。这些数据表明，b型流感嗜血杆菌菌株在细胞表面暴露蛋白方面存在抗原交叉反应。
