Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
EMBO J. 1984 Nov;3(11):2613-9. doi: 10.1002/j.1460-2075.1984.tb02182.x.
The structure of the tRNA-like 3' terminus of tobacco mosaic virus (TMV) RNA has been studied. A 3' -terminal fragment possessing the tRNA-like properties was probed with chemical modification and enzymatic digestions. A model of the secondary structure is proposed for the last 105 nucleotides. The corresponding region of other tobamoviral RNAs can be folded in an identical secondary structure. A three-dimensional model for the tRNA-like structure is given which is compared with those proposed earlier for the tRNA-like 3' termini of turnip yellow mosaic virus (TYMV) RNA and brome mosaic virus (BMV) RNA. A new building principle which we discovered previously by studying the latter RNAs appears to be applied twice in the tRNA-like structure of TMV RNA. The determination of the minimal length requirement for recognition of CTP, ATP:tRNA nucleotidyl-transferase reveals a size of 100 nucleotides in agreement with the models proposed.
烟草花叶病毒（TMV）RNA 的 tRNA 样 3'末端的结构已被研究。用化学修饰和酶消化探测具有 tRNA 样性质的 3'末端片段。提出了最后 105 个核苷酸的二级结构模型。其他烟草花叶病毒 RNA 的相应区域可以折叠成相同的二级结构。给出了 tRNA 样结构的三维模型，并与先前提出的芜菁黄花叶病毒（TYMV）RNA 和雀麦花叶病毒（BMV）RNA 的 tRNA 样 3'末端的模型进行了比较。我们之前在研究后两种 RNA 时发现的一个新的构建原则似乎在 TMV RNA 的 tRNA 样结构中应用了两次。最小长度要求的确定对于识别 CTP、ATP：tRNA 核苷酸转移酶具有重要意义，这表明其大小为 100 个核苷酸，与提出的模型一致。
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