Prince A M, Ikram H, Hopp T P
Proc Natl Acad Sci U S A. 1982 Jan;79(2):579-82. doi: 10.1073/pnas.79.2.579.
On the basis of theoretical considerations, a peptide (H peptide) was synthesized by Hopp and Woods [Hopp, T. P. & Woods, K. R. (1981) Proc. Natl. Acad. Sci. USA 78, 3824---3828]. This peptide contains a sequence of six amino acids postulated to represent the major epitope, or antibody-combining site, of hepatitis B virus surface antigen (HBsAg). We have used passive hemagglutination inhibition with monospecific antibodies against the a, d, and y subdeterminants of this antigen and against human serum albumin to investigate the antigenic specificities on this peptide, and we have found it to contain the HBsAg/a and HBsAg/d but not HBsAg/y or human serum albumin subdeterminants. When the peptide was conjugated onto human erythrocytes and injected into mice, it induced the formation of anti-HBsAg with and without the use of Freund's adjuvant. If anti-HBsAg/a confers immunity to infection with hepatitis B virus, as is generally thought, these findings may permit the development of a synthetic vaccine lacking all unnecessary antigenic determinants.
基于理论上的考虑，霍普和伍兹合成了一种肽（H肽）[霍普，T.P. & 伍兹，K.R.（1981年）《美国国家科学院院刊》78卷，3824 - 3828页]。这种肽包含一段由六个氨基酸组成的序列，据推测它代表乙肝病毒表面抗原（HBsAg）的主要表位或抗体结合位点。我们利用针对该抗原的a、d和y亚决定簇以及人血清白蛋白的单特异性抗体进行被动血凝抑制试验，以研究此肽上的抗原特异性，结果发现它含有HBsAg/a和HBsAg/d亚决定簇，但不含有HBsAg/y或人血清白蛋白亚决定簇。当将该肽与人类红细胞结合并注射到小鼠体内时，无论是否使用弗氏佐剂，它都能诱导抗HBsAg的形成。如果如人们普遍认为的那样，抗HBsAg/a能赋予对乙肝病毒感染的免疫力，那么这些发现可能会促成一种不含所有不必要抗原决定簇的合成疫苗的研发。
