利用猿猴病毒40载体在细胞培养中表达乙型肝炎病毒表面抗原基因。
Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector.
作者信息
Moriarty A M, Hoyer B H, Shih J W, Gerin J L, Hamer D H
出版信息
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2606-10. doi: 10.1073/pnas.78.4.2606.
Abstract
We have constructed a simian virus 40 recombinant carrying a fragment of DNA from hepatitis B virus. Cultured monkey kidney cells infected with this recombinant produce hepatitis B surface antigen. The antigen is excreted into the culture medium as 22-nm particles with the same physical properties, antigenic composition, and constituent polypeptides as those found in the sera of patients with type B hepatitis.
摘要
我们构建了一种携带乙肝病毒DNA片段的猴病毒40重组体。用这种重组体感染的培养猴肾细胞可产生乙肝表面抗原。该抗原以22纳米颗粒的形式分泌到培养基中，其物理性质、抗原组成和组成多肽与乙肝患者血清中的相同。
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