Richardson L, Weaker F J, Sheridan P J
Stain Technol. 1982 Jan;57(1):19-22. doi: 10.3109/10520298209066515.
The commonly used silver stains were found to be unsatisfactory for nervous tissue processed for autoradiography. A silver impregnation procedure for central nervous system tissues prepared for the autoradiographic study of steroid receptors is described. The procedure is a combination of several silver and reticular strains made up in solutions containing dimethylsulfoxide. The technique clearly distinguishes perikarya of neurons, brain nuclei and fiber tracts without substantial loss of silver grains, and thus greatly facilitates the identification of steroid receptor nuclei at all levels of the central nervous system.
人们发现，常用的银染法对于为放射自显影而处理的神经组织并不适用。本文描述了一种用于中枢神经系统组织的银浸染程序，该组织是为类固醇受体的放射自显影研究而制备的。该程序是几种银染法和网状染色法的组合，这些方法在含有二甲基亚砜的溶液中配制而成。该技术能够清晰地区分神经元的胞体、脑核和纤维束，且银颗粒基本不会损失，因此极大地便于在中枢神经系统的各个层面识别类固醇受体核。
