从豚鼠和兔胃中分离出的分散黏膜细胞分泌内因子。

Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach.

作者信息

Batzri S, Harmon J W, Walker M D, Thompson W F, Toles R

出版信息

Biochim Biophys Acta. 1982 Apr 29;720(2):217-21. doi: 10.1016/0167-4889(82)90015-5.

Abstract

In dispersed mucosal cells prepared from rabbit and guinea pig stomach, the secretion of intrinsic factor was constant (0.3-0.4%/min) for at least 30 min incubation at 37 degrees C. Histamine or isobutyl methylxanthine increased cyclic AMP and intrinsic factor secretion in both cells preparations. Isobutyl methylxanthine potentiated and cimetidine competitively inhibited (ki = 5.10-7 M) both effects of histamine. Dibutyryl cyclic AMP (1.0 mM), also caused a 3-fold increase in intrinsic factor secretion. These results suggest that in rabbit and guinea pig histamine interacts with H2-receptors to increase cyclic AMP which mediates the rise in the rate of intrinsic factor secretion.

摘要

在从兔和豚鼠胃制备的分散黏膜细胞中，在37℃孵育至少30分钟时，内因子的分泌是恒定的（0.3 - 0.4%/分钟）。组胺或异丁基甲基黄嘌呤可增加两种细胞制剂中的环磷酸腺苷（cAMP）和内因子分泌。异丁基甲基黄嘌呤增强了组胺的作用，而西咪替丁竞争性抑制（Ki = 5.10 - 7 M）组胺的这两种作用。二丁酰环磷酸腺苷（1.0 mM）也使内因子分泌增加了3倍。这些结果表明，在兔和豚鼠中，组胺与H2受体相互作用以增加环磷酸腺苷，环磷酸腺苷介导内因子分泌速率的升高。