Biddison W E, Kostyu D D, Strominger J L, Krangel M S
J Immunol. 1982 Aug;129(2):730-4.
Cytotoxic T cell (CTL) recognition of influenza virus in conjunction with HLA-A2 was examined in a population study. Virus-infected target cells from three unrelated A2-positive donors were not lysed by virus-immune CTL from any donor matched only for A2. The A2 antigens of these three donors were indistinguishable from the A2 antigens of other A2-positive donors as assessed by extensive serologic analyses; however, isoelectric focusing (IEF) of A2 molecules from these three donors demonstrated that their A2 heavy polypeptide chains are structurally distinct from those of "normal" A2-positive donors. To date 11% of all A2-positive donors tested exhibited a "variant" A2-associated CTL restriction antigen, and IEF of A2 heavy chains from all "variant" A2-positive cells revealed structural differences in each of these polypeptides. These results suggest there may be considerably greater polymorphism of HLA-A gene products than has been revealed by current serologic techniques.
在一项群体研究中，对细胞毒性T细胞（CTL）结合HLA - A2识别流感病毒的情况进行了检测。来自三名不相关的A2阳性供体的病毒感染靶细胞，未被仅与A2匹配的任何供体的病毒免疫CTL裂解。通过广泛的血清学分析评估，这三名供体的A2抗原与其他A2阳性供体的A2抗原没有区别；然而，对这三名供体的A2分子进行等电聚焦（IEF）显示，它们的A2重多肽链在结构上与“正常”A2阳性供体的不同。到目前为止，在所有检测的A2阳性供体中，11%表现出“变异”的A2相关CTL限制性抗原，并且对所有“变异”A2阳性细胞的A2重链进行IEF分析显示，这些多肽中的每一种都存在结构差异。这些结果表明，HLA - A基因产物的多态性可能比目前血清学技术所揭示的要大得多。
