Engelhard V H, Benjamin C
Immunogenetics. 1983;18(5):461-73. doi: 10.1007/BF00364388.
Long-term murine cytotoxic T-cell clones arising in response to stimulation with human lymphoblastoid cells and reactive with the HLA-A2 antigen are characterized. These clones distinguish between HLA-A2 and 21 other serologically defined HLA-A and -B antigens. In addition, most clones discriminate between prototypical HLA-A2 antigens, expressed by the majority of HLA-A2-positive individuals, and variant HLA-A2 antigens, which are serologically identical with the prototype, but distinguishable by human cytotoxic T cells and by biochemical analysis. This discrimination is reflected as an inability to cause any significant lysis of variant HLA-A2-expressing target cells at effector-to-target ratios 10- to 100-fold greater than those giving 50% lysis of prototype HLA-A2-expressing cells. By screening a panel of serologically HLA-A2-positive cells, a new variant HLA-A2-expressing cell line has been defined. The recognition patterns of these xenogeneic clones are suggested to reflect recognition of alloantigenic polymorphic determinants. Based on the strong bias in the xenogeneic T-cell repertoire for such determinants, we propose a model for T-cell recognition of class I products of the major histocompatibility complex.
对因用人淋巴母细胞刺激而产生并与HLA - A2抗原反应的长期鼠细胞毒性T细胞克隆进行了表征。这些克隆能够区分HLA - A2与其他21种血清学定义的HLA - A和 - B抗原。此外，大多数克隆能够区分大多数HLA - A2阳性个体所表达的典型HLA - A2抗原和变异HLA - A2抗原，这些变异抗原在血清学上与原型相同，但可被人细胞毒性T细胞和生化分析区分开来。这种区分表现为，在效应细胞与靶细胞比例比使表达原型HLA - A2的细胞发生50%裂解的比例高10至100倍时，无法对表达变异HLA - A2的靶细胞造成任何显著的裂解。通过筛选一组血清学上HLA - A2阳性的细胞，定义了一种新的表达变异HLA - A2的细胞系。这些异种克隆的识别模式被认为反映了对同种异体抗原多态性决定簇的识别。基于异种T细胞库中对此类决定簇的强烈偏向性，我们提出了一个主要组织相容性复合体I类产物的T细胞识别模型。
