Dubertret L, Breton-Gorius J, Fosse M, Touraine R
J Invest Dermatol. 1982 Jun;78(6):477-81. doi: 10.1111/1523-1747.ep12510235.
Intubation of unfixed and unfrozen slices of skin in diaminobenzidine allows visualization of a peroxidatic activity in perinuclear envelope of suprabasal keratinocytes undergoing orthokeratotic differentiation. Basal keratinocytes and melanocytes are always negative. This enzyme is absent in mucous and parakeratotic (psoriatic) differentiation. Mitochondria are also strongly stained by this technique and it was shown that the number of epidermal mitochondria is greatly increased in psoriatic lesions.
在二氨基联苯胺中对未固定和未冷冻的皮肤切片进行插管，可使正角化分化的基底上层角质形成细胞的核周膜呈现过氧化物酶活性。基底角质形成细胞和黑素细胞始终呈阴性。该酶在黏液和不全角化（银屑病）分化中不存在。线粒体也被该技术强烈染色，并且已表明银屑病皮损中表皮线粒体的数量大大增加。
