植物中姐妹染色单体交换频率的测定：一种用于蚕豆的简易福尔根染色程序。
Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for vicia faba.
作者信息
Tempelaar M J, de Both M T, Versteegh J E
出版信息
Mutat Res. 1982 Mar;103(3-6):321-6. doi: 10.1016/0165-7992(82)90061-6.
PMID:6178023
Abstract
A relatively straightforward approach is described to obtain differential contrast in sister chromatids for SCE detection in Vicia faba after BrdUrd incorporation. The hydrolysis time of the well-known Feulgen reaction was extended to differentially degrade the DNA, the BrdUrd-substituted strands being more resistant. The procedure may easily be adapted for other plant species with large chromosomes.
摘要
本文描述了一种相对简单的方法，用于在蚕豆中掺入BrdUrd后获得姐妹染色单体的差异对比度，以检测姐妹染色单体交换（SCE）。将著名的福尔根反应的水解时间延长，以差异性地降解DNA，而掺入BrdUrd的链更具抗性。该方法可轻松适用于其他具有大染色体的植物物种。
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