Soluble HLA antigens in normal human immunoglobulin preparations.

Nine lots of normal human immunoglobulin produced in Bulgaria and India, prepared from venous or placental blood, were assayed for the presence of HLA antigens by the inhibition of cytotoxicity of HLA alloantisera using two different absorption procedures. The results indicate that the normal human immunoglobulin preparations tested here contain soluble HLA antigens as demonstrated by the inhibition of HLA alloantisera. Most of the higher frequency antigens of the HLA-A, B, C and DR locus appear to be present in the Ig-preparations. In samples from India there appears to be a lack of HLA-A3 antigen, which corresponds to the low frequency of A3 in this population. Immunoglobulins produced from different raw materials contain different amounts of soluble HLA antigens.