在人免疫球蛋白制剂中未检测到可溶性HLA抗原。

Soluble HLA antigens not detected in human immunoglobulin preparations.

作者信息

McLean I L, Faed J M, Milligan L M, Adams D D

出版信息

Tissue Antigens. 1983 Jul;22(1):59-61. doi: 10.1111/j.1399-0039.1983.tb01166.x.

DOI:10.1111/j.1399-0039.1983.tb01166.x

Abstract

Immunoglobulin concentrates prepared from subjects of known HLA specificity were assayed for the presence of soluble HLA antigens by the inhibition of cytotoxic HLA alloantisera using a microabsorption procedure. The immunoglobulin preparations inhibited alloantisera of all specificities tested, regardless of the HLA type of the immunoglobulin donor, indicating that the observed inhibition was non-specific. Addition of extra complement reduced the inhibitory effects of the immunoglobulin concentrates. It is concluded that the inhibition of HLA alloantisera demonstrated by this technique is due to the anticomplementary activity of the immunoglobulin preparations, rather than their content of specific soluble HLA antigens.

摘要

使用微量吸收法，通过抑制细胞毒性 HLA 同种异体抗血清，对从已知 HLA 特异性个体制备的免疫球蛋白浓缩物进行可溶性 HLA 抗原检测。无论免疫球蛋白供体的 HLA 类型如何，这些免疫球蛋白制剂均能抑制所有测试特异性的同种异体抗血清，表明观察到的抑制是非特异性的。额外添加补体可降低免疫球蛋白浓缩物的抑制作用。得出的结论是，该技术所显示的 HLA 同种异体抗血清的抑制作用是由于免疫球蛋白制剂的抗补体活性，而非其特异性可溶性 HLA 抗原的含量。